14-3-3 σ Mouse Monoclonal Antibody (5D7)
Key features and details
- Reactivity: Human
- Application: WB, IP, IF, IHC(P), ELISA
- Host: Mouse
- Clonality: Monoclonal
- Isotype: IgG1 kappa light chain
- Target Name: 14-3-3 σ
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Brand:
CAT.NO. : ARA1457
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Product Details
BACKGROUND
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell cycle checkpoints. Seven iso - forms, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ, comprise this family of signaling intermediates. 14-3-3 σ, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominantly in stratified squamous keratinizing epithelium, 14-3-3 σ is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to KRT17, for example, 14-3-3 σ acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 σ also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 σ positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G₂/M cell cycle progression. Multiple isoforms of 14-3-3 σ exist due to alternative splicing events.
Application
14-3-3 σ (5D7) is recommended for detection of 14-3-3 σ of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100 - 1:1000), immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500), immunohistochemistry (including paraffin - embedded sections) (starting dilution 1:50, dilution range 1:50 - 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30 - 1:3000).
Data
14-3-3 σ (5D7). Western blot analysis of 14-3-3 σ expression in HeLa nuclear extract.
14-3-3 σ (5D7). Immunoperoxidase staining of formalin - fixed, paraffin - embedded human uterine cervix tissue showing nuclear and cytoplasmic localization.
SOURCE
Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
STORAGE
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
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