Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S
![Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S](/upload/image/products/Fig5_RM172-Colon-Cancer-20X.png)
![Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S](/upload/image/products/RM172_Fig1_1.png)
![Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S](/upload/image/products/RM166_Fig5_ICC.png)
![Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S](/upload/image/products/RM172_Fig3_ICC.png)
![Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM172]; H3K27ac; Histone H3 (K27 acetylation); 31-1056-00; 31-1056-00-S](/upload/image/products/RM172_Fig2.png)
Key features and details
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Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation) |
| Catalog No. | 31-1056-00 |
| Clone Name | RM172 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys27 Rabbit Monoclonal Antibody Data Sheet RM172 |
| Specificity | RM172 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex Immunohistochemistry (IHC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC:0.5 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. IHC: 0.5 µg/mL - 1 µg/mL |
| Keywords | |
| References for Acetyl-Histone H3 (Lys27) antibody [RM172] | Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854) Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794) Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153) Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279) |
 | RM172 specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3. |
 | Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM172 at 1 µg/mL, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys27) Rabbit mAb RM172 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using anti-Acetyl-Histone H3 (Lys27) rabbit monoclonal antibody, Clone RM172, at a 1:2000 dilution. |
| Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-Acetyl-Histone H3 (Lys27) rabbit monoclonal antibody, Clone RM172, at a 1:2000 dilution. |
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