Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/RM161_Fig2.png)
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/RM161-Fig1_1.png)
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/RM161-Fig3_e.png)
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/RM161-Fig4_e.png)
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/RM161_Fig5_ICC.png)
![Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM161]; H3K9ac; Histone H3 (K9 acetylation); ChIP Validated; 31-1054-00; 31-1054-00-S](/upload/image/products/HepG2_H3K9Ac_IHC.png)
Key features and details
- Uniprot:
- Gene ID:
- Host Species:
- Species Reactivity:
- Concentration:
-
Brand:
Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation) |
| Catalog No. | 31-1054-00 |
| Clone Name | RM161 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161 |
| Specificity | RM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9). |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) Immunohistochemistry (IHC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.25 µg/mL - 1 µg/mL; ICC:0.5 µg/mL - 2 µg/mL; ChIP: 2 µg/mL - 10 µg/mL; IHC: 0.1 µg/mL – 1 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.5 µg/mL. |
| Keywords | |
| References for Acetyl-Histone H3 (Lys9) antibody [RM161] | Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794) Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153) |
 | RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3. |
 | Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM161 at 0.25 µg/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa. |
 | Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 µg/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 µg/mL) as the detection antibody. |
 | ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red).Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemistry staining of HepG2 cells using anti-Acetyl-Histone H3 (Lys9) antibody, Clone RM161. |
New Products
