anti-Adiponectin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Adiponectin
  • 反应物种: Ms, Rat
  • 预测物种: Hu, Bov, Chk
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Adiponectin
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG22406
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Adiponectin
反应物种Ms, Rat
预测物种Hu, Bov, Chk
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Adiponectin
抗原物种Human
抗原Synthetic peptide around 15 aa (N-terminus) of Human Adiponectin.
偶联标记Un-conjugated
別名Adipose most abundant gene transcript 1 protein; ADPN; APM-1; Gelatin-binding protein; APM1; ACDC; Adiponectin; apM-1; ACRP30; ADIPQTL1; GBP28; 30 kDa adipocyte complement-related protein; Adipocyte, C1q and collagen domain-containing protein; Adipocyte complement-related 30 kDa protein
应用说明
应用建议
应用推荐稀释比
IHC-P1 µg/ml
WB1 - 4 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照WB: Rat brain cell lysate.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS and 0.02% Sodium azide.
抗菌剂0.02% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11450 Mouse ADIPOQ

GeneID: 246253 Rat ADIPOQ

Swiss-port # Q60994 Mouse Adiponectin

基因名称ADIPOQ
全名adiponectin, C1Q and collagen domain containing
背景介绍This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
生物功能Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. [UniProt]
预测分子量26 kDa
翻译后修饰Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes (By similarity).
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
检测图片 (2)
  • ARG22406 anti-Adiponectin antibody IHC-P image

    Immunohistochemistry: Rat brain tissue stained with ARG22406 anti-Adiponectin antibody at 1 µg/ml dilution.

  • ARG22406 anti-Adiponectin antibody WB image

    Western blot: Rat brain lysates stained with ARG22406 anti-Adiponectin antibody at 1, 2, and 4 µg/ml dilution.

参考文献

Maternal Separation Enhances Anti-contractile Perivascular Adipose Tissue Function in Male Rats on a High Fat Diet.

IHC-P / Rat

Loria AS et al.
Am J Physiol Regul Integr Comp Physiol.,  (2018)

publication_link

 

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