anti-beta Lactamase antibody [8A5.A10]
![anti-beta Lactamase antibody [8A5.A10]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
Key features and details
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- 靶点名称:
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CAT.NO. : ARG54472
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [8A5.A10] recognizes beta Lactamase |
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| 反应物种 | Bacteria |
| 应用 | ELISA |
| 特异性 | This antibody recognizes TEM-1-type beta-lactamases. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 8A5.A10 |
| 同位型 | IgG1 |
| 靶点名称 | beta Lactamase |
| 抗原物种 | E. coli |
| 抗原 | 5’-His-tagged E. coli 205 TEM-1 R+ beta-lactamase, accession no. P62593. Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW |
| 偶联标记 | Un-conjugated |
应用说明
| 应用说明 | Western blot: use at 10 ug/ml. Predicted molecular weight ~29kDa. ELISA: use at 10 - 20 ug/ml (optimized for beta - lactamase on solid phase at 10 ug/ml). These are recommended concentrations. Enduser should determine optimal concentrations for their application. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
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| 纯化 | Protein G-purified |
| 缓冲液 | PBS (pH 7.4) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to microbial production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys anti-bacterial activity. Bacteria the produce TEM- or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate specificity of these betalactamases. As a result, many of the beta-lactamase-producing Gram-negative bacteria have become multi-drug resistant. |
|---|---|
| 研究领域 | Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 32 kDa |
参考文献
克隆号文献
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