anti-Blood Group Lewis b antibody [2-25LE]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [2-25LE] recognizes Blood Group Lewis b
  • 反应物种: Hu
  • 应用: ELISA, IHC-P, WB
  • 特异性: The clone 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 2-25LE
  • 同位型: IgG1
  • 靶点名称: Blood Group Lewis b
  • Brand:
CAT.NO. : ARG62693
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Product Details
概述
产品描述Mouse Monoclonal antibody [2-25LE] recognizes Blood Group Lewis b
反应物种Hu
应用ELISA, IHC-P, WB
特异性The clone 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
宿主Mouse
克隆Monoclonal
克隆号2-25LE
同位型IgG1
靶点名称Blood Group Lewis b
抗原Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
IHC-P5 - 10 µg/ml
WBAssay-dependent
应用说明IHC-P: Heat mediated antigen retrieval (sodium citrate); mAb incubated for 1 hour at RT; Detection DAB
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照IHC-P: Colorectal adenocarcinoma
属性
形式Liquid
纯化Purified from hybridoma culture supernatant by protein A-affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
研究领域Cell Biology and Cellular Response antibody
检测图片 (2)
  • ARG62693 anti-Blood Group Lewis b antibody [2-25LE] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon adenocarcinoma tissue stained with ARG62693 anti-Blood Group Lewis b antibody [2-25LE].

  • ARG62693 anti-Blood Group Lewis b antibody [2-25LE] IHC-P image

    Immunohistochemistry: Human small intestine paraffin section stained with ARG62693 anti-Blood Group Lewis b antibody [2-25LE].

参考文献

克隆号文献

Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses.

Yu Y et al.
Mol Cell Proteomics.,  (2014)

publication_link

 

hr_line

Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells.

Zhuang H et al.
Biochimie.,  (2014)

publication_link

 

hr_line

Therapeutic targeting of Lewis(y) and Lewis(b) with a novel monoclonal antibody 692/29.

IHC-P, FACS / Human

Noble P et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors.

FACS / Human

Nordén R et al.
Glycobiology.,  (2013)

publication_link

 

hr_line

Functional glycomic analysis of human milk glycans reveals the presence of virus receptors and embryonic stem cell biomarkers.

Yu Y et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

Presence of terminal N-acetylgalactosamineβ1-4N-acetylglucosamine residues on O-linked oligosaccharides from gastric MUC5AC: involvement in Helicobacter pylori colonization?

WB / Human

Kenny DT et al.
Glycobiology.,  (2012)

publication_link

 

hr_line

Investigation of unexpected serum CA19-9 elevation in Lewis-negative cancer patients.

Hamada E et al.
Ann Clin Biochem.,  (2012)

publication_link

 

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