anti-CD152 / CTLA4 antibody [BNI3] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
反应物种: Hu
应用: FACS
特异性: The mouse monoclonal antibody BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
宿主: Mouse
克隆: Monoclonal
克隆号: BNI3
同位型: IgG2a
靶点名称: CD152 / CTLA4

CAT.NO. : ARG54228

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Size:

50 tests

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Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
反应物种	Hu
应用	FACS
特异性	The mouse monoclonal antibody BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
宿主	Mouse
克隆	Monoclonal
克隆号	BNI3
同位型	IgG2a
靶点名称	CD152 / CTLA4
抗原物种	Human
抗原	Human CD152-IgG heavy chain fusion protein
偶联标记	PE
別名	GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

应用说明

应用建议

应用	推荐稀释比
FACS	10 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1493 Human CTLA4 Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4
基因名称	CTLA4
全名	cytotoxic T-lymphocyte-associated protein 4
背景介绍	CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.
生物功能	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	25 kDa
翻译后修饰	N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

检测图片 (3)

ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) FACS image
Flow Cytometry: PHA stimulated human peripheral whole blood stained with ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) FACS image
Flow Cytometry: PHA stimulated human lymphocytes stained with ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) (10 µl reagent / 100 µl of peripheral whole blood) and ARG54302 anti-CD3 antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) FACS image
Flow Cytometry: Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed). Human PHA stimulated peripheral whole blood stained with ARG54228 anti-CD152 / CTLA4 antibody [BNI3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).