anti-CD33 antibody [WM53] (PerCP)
![anti-CD33 antibody [WM53] (PerCP)](/upload/image/products/ARG54315_FACS_1_201117_210_205.jpg)
![anti-CD33 antibody [WM53] (PerCP)](/upload/image/products/ARG54315_FACS_1_201117.jpg)
Key features and details
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CAT.NO. : ARG54315
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Product Details
概述
| 产品描述 | PerCP-conjugated Mouse Monoclonal antibody [WM53] recognizes CD33 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA IV; WS Code M-505 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | WM53 |
| 同位型 | IgG1 |
| 靶点名称 | CD33 |
| 抗原物种 | Human |
| 抗原 | Human AML cells |
| 偶联标记 | PerCP |
| 別名 | p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD33 |
| 全名 | CD33 molecule |
| 背景介绍 | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| 生物功能 | CD33: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323). [UniProt] |
| 产品亮点 | Related products: CD33 antibodies; CD33 ELISA Kits; CD33 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. |
检测图片 (1)
ARG54315 anti-CD33 antibody [WM53] (PerCP) FACS image
Flow Cytometry: Human peripheral blood stained with ARG54315 anti-CD33 antibody [WM53] (PerCP).
参考文献
克隆号文献
Highly multiparametric analysis by mass cytometry.
FACS / Human
A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.
Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.
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