anti-Collagen II antibody [5B2.5]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [5B2.5] recognizes Collagen II
  • 反应物种: Hu, Ms, Chk, Pig, Rb
  • 应用: ICC/IF, IHC-Fr, IHC-P, WB
  • 特异性: This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 5B2.5
  • 同位型: IgG2a, kappa
  • 靶点名称: Collagen II
  • Brand:
CAT.NO. : ARG62450
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Product Details
概述
产品描述Mouse Monoclonal antibody [5B2.5] recognizes Collagen II
反应物种Hu, Ms, Chk, Pig, Rb
应用ICC/IF, IHC-Fr, IHC-P, WB
特异性This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
宿主Mouse
克隆Monoclonal
克隆号5B2.5
同位型IgG2a, kappa
靶点名称Collagen II
抗原物种Chicken
抗原A purified preparation of lathyritic type II collagen from embryonic chicken sternum
偶联标记Un-conjugated
別名Collagen alpha-1(II) chain; Alpha-1 type II collagen
应用说明
应用建议
应用推荐稀释比
ICC/IF1/10 - 1/500
IHC-Fr1/10 - 1/500
IHC-P1/10 - 1/500
WB1 - 2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Protein A purified
缓冲液1X PBS buffer with 0.1% sodium azide.
抗菌剂0.1% sodium azide.
浓度2 mg/ml
存放说明Aliquot and store at 2-8°C. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 1280 Human COL2A1

GeneID: 12824 Mouse COL2A1

GeneID: 395069 Chicken COL2A1

基因名称COL2A1
全名collagen, type II, alpha 1
背景介绍The alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
生物功能Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [provided by Uniprot]
细胞定位Secreted
研究领域Developmental Biology antibody; Signaling Transduction antibody
预测分子量142 kDa
翻译后修饰Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.
检测图片 (1)
  • ARG62450 anti-Collagen II antibody [5B2.5] ICC/IF image

    Immunofluorescence: Rabbit NPSC stained with ARG62450 anti-Collagen II antibody [5B2.5].

    From Shan-Zheng Wang et al. Stem Cell Res Ther. (2018), doi: 10.1186/s13287-018-0937-7, Fig. 6I.

参考文献

Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for earlyintervertebral disc regeneration?

WB, ICC/IF / Rabbit

Wang Shan-Zheng et al.
Stem Cell Res Ther.,  (2018)

publication_link

 

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