anti-CPEB3 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CPEB3
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CPEB3
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 150-400 of Human CPEB3 (NP_055727.3).
  • Brand:
CAT.NO. : ARG40606
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CPEB3
反应物种Hu, Ms, Rat
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CPEB3
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 150-400 of Human CPEB3 (NP_055727.3).
偶联标记Un-conjugated
別名CPE-binding protein 3; CPE-BP3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:200 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Mouse heart
实际分子量72 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 208922 Mouse CPEB3

GeneID: 22849 Human CPEB3

Swiss-port # Q7TN99 Mouse Cytoplasmic polyadenylation element-binding protein 3

Swiss-port # Q8NE35 Human Cytoplasmic polyadenylation element-binding protein 3

基因名称CPEB3
全名cytoplasmic polyadenylation element binding protein 3
生物功能Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (By similarity). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure (By similarity). Required for the consolidation and maintenance of hippocampal-based long term memory (By similarity). In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation (By similarity). Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B (By similarity). When activated, acts as a translational activator of GRIA1 and GRIA2 (By similarity). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (By similarity). Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (By similarity). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (By similarity). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (PubMed:21336257). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (PubMed:20639532). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (PubMed:26398195). [UniProt]
细胞定位Cytoplasm. Nucleus. Cell junction, synapse. Cell projection, dendrite. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Predominantly cytoplasmic in unstimulated neurons but translocates to the nucleus following neuronal stimulation (PubMed:20639532, PubMed:22730302). Nuclear import is mediated by importin IPO5 (By similarity). [UniProt]
预测分子量76 kDa
翻译后修饰Activated by NEURL1-mediated monoubiquitination, resulting in the growth of new dendritic spines and increased levels of GRIA1 and GRIA2. NEURL1-mediated monoubiquitination facilitates synaptic plasticity and hippocampal-dependent memory storage.

Under basal unstimulated conditions when CPEB3 is mainly unaggregated, sumoylated and acts as a translational repressor. Following neuronal stimulation, becomes desumoylated and aggregated which is required for the translation of mRNA targets and for dendritic filopodia formation.

Following neuronal stimulation, cleaved by CAPN2 which abolishes its translational repressor activity, leading to translation of CPEB3 target mRNAs.

Phosphorylation is enhanced by neuronal stimulation. [UniProt]
检测图片 (1)
  • ARG40606 anti-CPEB3 antibody WB image

    Western blot: 25 µg of Mouse heart lysate stained with ARG40606 anti-CPEB3 antibody at 1:1000 dilution.

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