anti-GABAB Receptor 1 antibody [S93A-49]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [S93A-49] recognizes GABAB Receptor 1
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, WB
  • 特异性: Detects ~115kDa. No cross-reactivity against GABA(B)R2.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: S93A-49
  • 同位型: IgG1
  • 靶点名称: GABAB Receptor 1
  • Brand:
CAT.NO. : ARG22243
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Product Details
概述
产品描述Mouse Monoclonal antibody [S93A-49] recognizes GABAB Receptor 1
反应物种Hu, Ms, Rat
应用ICC/IF, WB
特异性Detects ~115kDa. No cross-reactivity against GABA(B)R2.
宿主Mouse
克隆Monoclonal
克隆号S93A-49
同位型IgG1
靶点名称GABAB Receptor 1
抗原物种Rat
抗原Fusion protein around aa. 873-977 (cytoplasmic C-terminus) of Rat GABA B Receptor 1
偶联标记Un-conjugated
別名Gb1; GABA-B-R1; GABA-B receptor 1; GABA-BR1; GPRC3A; GABBR1-3; dJ271M21.1.2; dJ271M21.1.1; GABABR1; Gamma-aminobutyric acid type B receptor subunit 1; GB1
应用说明
应用建议
应用推荐稀释比
ICC/IFAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂0.09% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2550 Human GABBR1

GeneID: 54393 Mouse GABBR1

GeneID: 81657 Rat GABBR1

基因名称Gabbr1
全名gamma-aminobutyric acid (GABA) B receptor 1
背景介绍Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jun 2009]
生物功能Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.

Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites. [UniProt]
细胞定位Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse
预测分子量108 kDa
检测图片 (2)
  • ARG22243 anti-GABAB Receptor 1 antibody [S93A-49] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22243 anti-GABAB Receptor 1 antibody [S93A-49] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Primary antibody (D) Composite.

  • ARG22243 anti-GABAB Receptor 1 antibody [S93A-49] WB image

    Western blot: Rat brain membrane lysate stained with ARG22243 anti-GABAB Receptor 1 antibody [S93A-49] at 1:1000 dilution.

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