anti-GNAT3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG43420
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GNAT3 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GNAT3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human GNAT3. |
| 偶联标记 | Un-conjugated |
| 別名 | GDCA; Guanine nucleotide-binding protein G(t) subunit alpha-3; Gustducin alpha-3 chain |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Mouse heart and Mouse muscle IHC-P: Human thyroid cancer and Human esophagus cancer | ||||||
| 实际分子量 | ~ 37 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 0.4 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # A8MTJ3 Human Guanine nucleotide-binding protein G(t) subunit alpha-3 Swiss-port # Q3V3I2 Mouse Guanine nucleotide-binding protein G(t) subunit alpha-3 |
|---|---|
| 基因名称 | GNAT3 |
| 全名 | guanine nucleotide binding protein, alpha transducing 3 |
| 背景介绍 | Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome. [provided by RefSeq, Dec 2015] |
| 生物功能 | Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane. [UniProt] |
检测图片 (3)
ARG43420 anti-GNAT3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG43420 anti-GNAT3 antibody (left) at 1:35 dilution, or the same antibody pre-incubated with synthetic peptide (right). (Original magnification: X200).
ARG43420 anti-GNAT3 antibody WB image
Western blot: 40 µg of Mouse heart and Mouse muscle lysate stained with ARG43420 anti-GNAT3 antibody at 1:400 dilution.
ARG43420 anti-GNAT3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophagus cancer tissue stained with ARG43420 anti-GNAT3 antibody (left) at 1:35 dilution, or the same antibody pre-incubated with synthetic peptide (right). (Original magnification: X200).
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