anti-Huntingtin antibody [HDB4E10]
![anti-Huntingtin antibody [HDB4E10]](/upload/image/products/ARG22448_WB_1_210_205.jpg)
![anti-Huntingtin antibody [HDB4E10]](/upload/image/products/ARG22448_WB_1.jpg)
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CAT.NO. : ARG22448
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [HDB4E10] recognizes Huntingtin This antibody recognizes with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein. Mouse anti Huntingtin antibody, clone HDB4E10 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. The combined use of Mouse anti Huntingtin antibody, clones HDB4E10, HDC8A4 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain (Jones 1999). |
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| 反应物种 | Hu, Ms, Rb |
| 应用 | IHC-Fr, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HDB4E10 |
| 同位型 | IgG1 |
| 靶点名称 | Huntingtin |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin. |
| 偶联标记 | Un-conjugated |
| 別名 | Huntingtin; Huntington disease protein; HD protein; IT15; HD |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | HTT |
| 全名 | huntingtin |
| 背景介绍 | Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play a role in microtubule-mediated transport or vesicle function. [UniProt] |
| 预测分子量 | 348 kDa |
| 翻译后修饰 | Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis. Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. |
检测图片 (1)
ARG22448 anti-Huntingtin antibody [HDB4E10] WB image
Western blot: Normal Human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and stained with ARG22448 anti-Huntingtin antibody [HDB4E10].
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