anti-Intra Acrosomal Protein antibody [Hs-14]
![anti-Intra Acrosomal Protein antibody [Hs-14]](/upload/image/products/Hs-14_1_210_205.jpg)
![anti-Intra Acrosomal Protein antibody [Hs-14]](/upload/image/products/Hs-14_1.jpg)
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CAT.NO. : ARG63043
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [Hs-14] recognizes Intra Acrosomal Protein |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | The clone Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle._x000D_ |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Hs-14 |
| 同位型 | IgM |
| 靶点名称 | Intra Acrosomal Protein |
| 抗原物种 | Human |
| 抗原 | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| 偶联标记 | Un-conjugated |
| 別名 | D11S4365; Acrosomal vesicle protein 1; SP-10; Acrosomal protein SP-10; SPACA2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | ICC/IF: Staining technique: Membrane permeabilization (acetone) is essential. Application note: The clone Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by precipitation methods and size-exclusion chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACRV1 |
| 全名 | acrosomal vesicle protein 1 |
| 背景介绍 | One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
检测图片 (1)
ARG63043 anti-Intra Acrosomal Protein antibody [Hs-14] ICC/IF image
Immunofluorescence: Acetone-permeabilized human sperms sample stained with ARG63043 anti-Intra Acrosomal Protein antibody [Hs-14].
参考文献
克隆号文献
Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.
[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization].
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