anti-Notch 3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes Notch 3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | FACS, IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Notch 3 |
| 抗原物种 | Human |
| 抗原 | C-QLGPQPEVTPKRQ |
| 偶联标记 | Un-conjugated |
| 別名 | LMNS; CADASIL; CASIL; Neurogenic locus notch homolog protein 3; IMF2; Notch 3 |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # Q9UM47 Human Neurogenic locus notch homolog protein 3 |
|---|---|
| 背景介绍 | This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 244 kDa |
| 翻译后修饰 | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). Phosphorylated. Hydroxylated by HIF1AN. |
ARG63870 anti-Notch 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63870 anti-Notch 3 antibody at 5 µg/ml dilution followed by AP-staining.
ARG63870 anti-Notch 3 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Hippocampus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63870 anti-Notch 3 antibody at 4 µg/ml dilution followed by HRP-staining.
ARG63870 anti-Notch 3 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63870 anti-Notch 3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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