anti-PCSK1 / PC1/3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes PCSK1 / PC1/3 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PCSK1 / PC1/3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 726-740 of Human PCSK1 / PC1/3. (DVFYNTKPYKHRDDR) |
| 偶联标记 | Un-conjugated |
| 別名 | Proprotein convertase 1; EC 3.4.21.93; Neuroendocrine convertase 1; PC3; BMIQ12; PC1; Prohormone convertase 1; NEC1; NEC 1; SPC3 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 85 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | PCSK1 |
| 全名 | proprotein convertase subtilisin/kexin type 1 |
| 背景介绍 | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014] |
| 生物功能 | Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. [UniProt] |
| 细胞定位 | Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules. [UniProt] |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | O-glycosylated. [UniProt] |
ARG42953 anti-PCSK1 / PC1/3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42953 anti-PCSK1 / PC1/3 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42953 anti-PCSK1 / PC1/3 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat liver, Rat thymus, A549, HeLa, COLO320 and PANC cell lysates stained with ARG42953 anti-PCSK1 / PC1/3 antibody at 0.5 µg/ml dilution, overnight at 4°C.
ARG42953 anti-PCSK1 / PC1/3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42953 anti-PCSK1 / PC1/3 antibody at 1 µg/ml dilution, overnight at 4°C.
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