anti-PIP4K2A antibody
CAT.NO. : ARG55715
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概述
产品描述 | Rabbit Polyclonal antibody recognizes PIP4K2A |
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反应物种 | Hu |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | PIP4K2A |
抗原物种 | Human |
抗原 | KLH-conjugated synthetic peptide corresponding to aa. 329-363 (C-terminus) of Human PIP4K2A. |
偶联标记 | Un-conjugated |
別名 | PtdIns; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; 4; PIP4KII-alpha; Diphosphoinositide kinase 2-alpha; EC 2.7.1.149; 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; PIP5K2A; 5; PIPK; PIP5KIII; PI5P4KA; Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; PI; PIP5KIIA; PIP5KII-alpha |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
阳性对照 | Human brain |
属性
形式 | Liquid |
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纯化 | Purification with Protein A and immunogen peptide. |
缓冲液 | PBS and 0.09% (W/V) sodium azide. |
抗菌剂 | 0.09% (W/V) sodium azide. |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | Swiss-port # P48426 Human Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha |
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基因名称 | PIP4K2A |
全名 | phosphatidylinositol-5-phosphate 4-kinase, type II, alpha |
背景介绍 | Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008] |
生物功能 | Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. [UniProt] |
细胞定位 | Cell membrane. Nucleus. Cytoplasm. Note=May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light (By similarity). Localization to the nucleus is modulated by the interaction with PIP4K2B. |
预测分子量 | 46 kDa |
检测图片 (3)
ARG55715 anti-PIP4K2A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG55715 anti-PIP4K2A antibody at 1:25 dilution.
ARG55715 anti-PIP4K2A antibody WB image
Western blot: 20 µg of Human brain lysate stained with ARG55715 anti-PIP4K2A antibody at 1:1000 dilution.
ARG55715 anti-PIP4K2A antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55715 anti-PIP4K2A antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
