anti-PKC alpha antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG43932
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PKC alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PKC alpha |
| 抗原物种 | Human |
| 抗原 | Human PKC alpha recombinant protein |
| 表达系统 | E.coli |
| 偶联标记 | Un-conjugated |
| Protein Full name | Protein kinase C alpha type |
| 別名 | PRKCA; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PKCalpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCI+/-; PKCα; AAG6 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRKCA |
| 全名 | Protein Kinase C Alpha |
| 背景介绍 | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| 生物功能 | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4+ T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42. |
| 细胞定位 | Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus |
| 预测分子量 | 76 kDa |
| 翻译后修饰 | Acetylation, Phosphoprotein |
检测图片 (4)
ARG43932 anti-PKC alpha antibody WB image
Western blot: HeLa stained with ARG43932anti-PKC alpha antibody at 0.5 μg/mL dilution.
ARG43932 anti-PKC alpha antibody FACS image
Flow Cytometry: SiHa cells stained withARG43932 anti-PKC alpha antibody (blue) at 1 μg/1x10^6 cells dilution.
ARG43932 anti-PKC alpha antibody WB image
Western blot: C6 stained with ARG43932 anti-PKC alpha antibody at 0.5 μg/mL dilution.
ARG43932 anti-PKC alpha antibody WB image
Western blot: NIH/3T3 stained with ARG43932 anti-PKC alpha antibody at 0.5 μg/mL dilution.
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