Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM175]; H3K27me3; Histone H3 (trimethyl K27); ChIP Validated; 31-1062-00; 31-1062-00-S
![Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM175]; H3K27me3; Histone H3 (trimethyl K27); ChIP Validated; 31-1062-00; 31-1062-00-S](/upload/image/products/RM175-Fig2.png)
![Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone RM175]; H3K27me3; Histone H3 (trimethyl K27); ChIP Validated; 31-1062-00; 31-1062-00-S](/upload/image/products/RM175-Fig1_E.png)
Key features and details
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Product Details
| Product Name | Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27) |
| Catalog No. | 31-1062-00 |
| Clone Name | RM175 |
| Product Data Sheet | Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175 |
| Specificity | RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3. |
| Immunogen | A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27). |
| Application | Chromatin IP (ChIP) Western Blot (WB) Immunohistochemistry (IHC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ChIP: 1 µg - 5 µg; Western Blot: 1 µg/mL – 2 µg/mL; IHC: 0.5 µg/mL – 2.5 µg/mL; ELISA: 0.5 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. |
| . | |
 | RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 µg/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human seminoma tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human glioblastoma tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human tumor tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175, from user review. |
| References for Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27) [RM175] | Andrea J Kriz et al. Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation Mol Cell (IF: 17.97; Q1) . 2021 May 6;81(9):1970-1987. ChIP; Read more (PMID: 33725485) Chia-Chi Tsai et al. Overexpression of Histone H3 Lysine 27 Trimethylation Is Associated with Aggressiveness and Dedifferentiation of Thyroid Cancer Endocr Pathol 2019 Dec;30(4):305-311.Human IHC Read more (PMID: 31396854) Chen-Yu Wang et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments Nat Commun (IF: 14.92; Q1). 2019 Jul 3;10(1):2950. ICC/IF ; Read more (PMID: 31270318) Peitao Lü et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. Nat Plants (IF: 13.26; Q1). 2018 Oct;4(10):784-791 ChIP;Read more (PMID: 30250279) Chen-Yu Wang et al. SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X Cell (IF: 41.58; Q1). 2018 Jul 12;174(2):406-421. ICC/IF ; Read more (PMID: 29887375) |
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