BAX Rabbit Monoclonal Antibody(ARA865)

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. BAX is a member of the Bcl-2 gene family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. BAX is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence also suggests that activated BAX and/or Bak form an oligomeric pore, MAC in the MOM (mitochondrial outer membrane). This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases. This defines a direct role for BAX in mitochondrial outer membrane permeabilization. BAX activation is stimulated by various abiotic factors, including heat, hydrogen peroxide, low or high pH, and mitochondrial membrane remodeling. In addition, it can become activated by binding BCL-2, as well as non-BCL-2 proteins such as p53 and Bif-1. Conversely, BAX can become inactivated by interacting with VDAC2, Pin1, and IBRDC2.

Application	Dilution Ratio
IHC-P	1:200

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human Bax aa 50-150 (internal sequence)
Species Reactivity	Human
Validated Applications	IHC-P
Molecular Weight	Predicted band size: 21 kDa
Positive Control	Human breast tissue, human cervical carcinoma tissue, human kidney tissue
Conjugation	unconjugated
RRID	AB_3072441
Form	Liquid
Concentration	1μg/ul
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with BAX Rabbit Monoclonal Antibody(ARA865)

Immunohistochemical analysis of paraffin-embedded human kidney tissue with BAX Rabbit Monoclonal Antibody(ARA865)

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

For Research Use Only. Not for use in diagnostic procedures.