CSRP1 Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse, Rat, Chicken
- Application:WB, IHC, IF/ICC
- Host:Rabbit
- Clonality:Polyclonal
- Target:CSRP1
-
Brand:
CAT.NO. : ARA6874
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Product Details
Background
This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this gene product occurs in proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Alternatively spliced transcript variants have been described.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:50 - 1:100 |
|
IF/ICC |
1:50 - 1:200 |
Overview
Data
Western blot analysis of CSRP1 expression in Jurkat (A), HeLa (B), PC3 (C), DU145 (D), A10 (E) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 23 kD)
Immunohistochemical analysis of CSRP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CSRP1 staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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