ER Rabbit Monoclonal Antibody(ARB845)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6635
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Product Details
Background
Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems.
Studies have shown ER α is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas.
Studies with immunohistochemical assay show that positive steroid hormone status has predicted favourable overall, survival, independently of hormonal treatment. Secondly, ER α can be used as a tumour marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.
Studies have shown ER α is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas.
Studies with immunohistochemical assay show that positive steroid hormone status has predicted favourable overall, survival, independently of hormonal treatment. Secondly, ER α can be used as a tumour marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.
Overview
| Target | Estrogen Receptor α |
| Host Species | rabbit monoclonal antibody |
| Molecular Weight | 66 kDa |
| Purity | ProA affinity purified IgG |
| Species Cross-reactivity | Human |
| Form | Liquid |
| Applications | IHC-P |
| Swissprot ID | P03372 |
| Immunogen | Synthetic peptide within aa495-595 of ER alpha |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
| Storage Conditions | -25°C to -18°C |
| Dilutions | IHC-P: 1:100-1:200 |
| Subcellular Location | Nucleus |
| Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 minutes |
Data
Immunohistochemical staining of human breast carcinoma tissue sections negative for estrogen receptor (ER) using ER Rabbit Monoclonal Antibody (ARB845).
Immunohistochemical staining of human breast carcinoma tissue sections positive for estrogen receptor (ER) using ER Rabbit Monoclonal Antibody (ARB845).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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