ERK1 Rabbit Monoclonal Antibody(ARA780)

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.

Application	Dilution Ratio
WB	1:2,000 - 1:10,000
IHC-P	1:100 - 1:200
IP	1:10 - 1:50
FC	1:200 - 1:1,000
ICC/IF	1:50 - 1:200

Predicted Molecular Weight	43kDa
Species Cross-reactivity	Human, Mouse, Rat (Species cross-reactivity determined by WB)
Validated Applications	WB, IHC-P, IF/ICC, FC, IP
Purity	ProA affinity purified IgG
Form	Liquid
Swissprot ID	P27361
Immunogen	A synthetic peptide corresponding to the N-term of ERK1 was used as an immunogen.
Storage Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.

All lanes: Anti-ERK1 antibody at 1:2,000 dilution
Predicted MW: 43 kDa
Observed MW: 44 kDa
Lane 1: Hela
Lane 2: 293T
Lane 3: Raw264.7
Lane 4: A431
Lane 5: A375
Lysate at 10 μg per lane
2nd Ab: GAR HRP(H+L) 1:5,000
Exposure: 50s

All lanes: Anti-ERK1 antibody at 1:2,000 dilution
Predicted MW: 43 kDa
Observed MW: 44 kDa
Lane 1: Mu Brain
Lane 2: Mu Kidney
Lane 3: Mu Liver
Lane 4: Rat Brain
Lane 5: Rat Kidney
Lysate at 10 μg per lane
2nd Ab: GAR HRP(H+L) 1:5,000
Exposure: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin paraffin-embedded sections) analysis Cervix cancer tissue labelling ERK1 with ERK1 Rabbit Monoclonal Antibody(ARA780)

ERK1 Rabbit Monoclonal Antibody(ARA780) staining ERK1 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature.
Samples were incubated with primary antibody (1:50) at 4°C.
An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500).
DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).

Overlay histogram showing Hela cells stained with ERK1 Rabbit Monoclonal Antibody(ARA780) (Red).
The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min.
The cells were then incubated in the antibody (ERK1 Rabbit Monoclonal Antibody(ARA780), 1:1,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature.
The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature.
Unlabelled sample (Black) was used as a control.

ERK1 was immunoprecipitated from 0.4mg of A375 whole cell lysate with ERK1 Rabbit Monoclonal Antibody(ARA780) at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ERK1 Rabbit Monoclonal Antibody(ARA780) IP in A375 whole cell lysate
Lane 2: PBS instead of ERK1 Rabbit Monoclonal Antibody(ARA780) in A375 whole cell lysate
Lane 3: A375 whole cell lysate, 10 μg (input)
Exposure: 120s

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

For Research Use Only. Not for use in diagnostic procedures.