Factor VIII Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human,Dog
- Application:WB,IHC
- Host:Rabbit
- Clonality:Polyclonal
- Target:Factor VIII
-
Brand:
CAT.NO. : ARA6625
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Size:
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Product Details
Background
This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:50 - 1:100 |
Overview
|
Immunogen |
KLH - conjugated synthetic peptide encompassing a sequence within the C - term region of human Factor VIII. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Polyclonal |
|
Product Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Name |
F8 |
|
Related Names |
F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component |
|
Gene ID (Human) |
2157 |
|
Protein ID (Human) |
P00451 |
Data
Western blot analysis of Factor VIII expression in HeLa (A), HepG2 (B) whole cell lysates. (Predicted band size: 267 kD; Observed band size: 280 kD)
Immunohistochemical analysis of Factor VIII staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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