Human Asprosin ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0032
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Size:
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Product Details
Background
Asprosin is a new type of adipocytokine discovered by Romere et al. in 2016. It is mainly produced and secreted by white adipocytes, which can bind to the receptors on the surface of hepatocytes on the one hand, stimulate the glucose output of hepatocytes, and directly increase blood sugar; On the other hand, it can penetrate the blood-brain barrier, activate the neuronal activity of agRP gene-associated protein (AgRP) associated with feeding-related in the hypothalamic center, and inhibit the neuronal activity of anorexia-related opioid-melanocyte corticogen (POMC), promote food intake, and indirectly exert a hypoglycemic effect.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0407 | 0.0468 | 0.0438 | |
| 4.69 | 0.1037 | 0.1032 | 0.1035 | 0.0597 |
| 9.38 | 0.1406 | 0.1638 | 0.1522 | 0.1085 |
| 18.75 | 0.2698 | 0.2895 | 0.2797 | 0.2359 |
| 37.50 | 0.5256 | 0.5766 | 0.5511 | 0.5074 |
| 75.00 | 1.0950 | 1.1600 | 1.1275 | 1.0838 |
| 150.00 | 2.3230 | 2.0280 | 2.1755 | 2.1318 |
| 300.00 | 3.6020 | 3.3780 | 3.4900 | 3.4463 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 6.4 | 29.6 | 112.8 | 5.9 | 31.2 | 118.1 |
| Standard Deviation | 0.3 | 1.2 | 5.9 | 0.3 | 1.3 | 5.4 |
| Coefficient of Variation(%) | 4 | 4 | 5.2 | 5.1 | 4.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 3.35-128.74 | 100 | 34.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Asprosin in this assay. No medical histories were available for the donors.
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