Human CD273/B7-DA/PD-L2 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.01 ng/ml (50 μl)；0.10 ng/ml (10 μl)；
Standard Curve Range: 0.14~100 ng/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 75min

Brand:

CAT.NO. : AEHY0011

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Size:

96T

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Product Details

Background

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

typical data

ng/ml	O.D.		Average	Corrected
0.00	0.0077	0.0086	0.0082
0.14	0.0225	0.0239	0.0232	0.0151
0.41	0.0562	0.0586	0.0574	0.0493
1.23	0.1686	0.1711	0.1699	0.1617
3.70	0.5579	0.5243	0.5411	0.5330
11.11	1.6230	1.5880	1.6055	1.5974
33.33	3.4830	3.5050	3.4940	3.4859
100.00	4.3062	4.3968	4.3515	4.3434

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（ng/ml）	2.0	10.6	29.4	1.8	10.1	29.2
Standard deviation	0.1	0.6	1.4	0.1	0.5	1.5
Coefficient of variation（%）	2.7	5.3	4.9	5.2	5.4	5.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

spike recovery

The spike recovery was evaluated by spiking 3 levels of human CD273/B7-DC/PD-L2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 120% with an overall mean recovery of 105%.

sample values

Sample Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

5.48-15.01

100

9.93

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human CD273/B7-DC/PD-L2 in this assay. No medical histories were available for the donors.

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