Human ICAM-1/CD54 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.22 pg/ml (50 μl)；0.05 ng/ml (10 μl)
Standard Curve Range: 0.14~100 ng/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 75min

Brand:

CAT.NO. : AEHY0008

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Size:

96T

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Product Details

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

Typical data

ng/ml	O.D.		Average	Corrected
0.00	0.0050	0.0046	0.0048
0.14	0.0210	0.0195	0.0203	0.0155
0.41	0.0519	0.0540	0.0530	0.0482
1.23	0.1489	0.1465	0.1477	0.1429
3.70	0.4238	0.4106	0.4172	0.4124
11.11	1.0740	1.0970	1.0855	1.0807
33.33	2.3350	2.3240	2.3295	2.3247
100.00	3.1570	3.3020	3.2295	3.2247

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（ng/ml）	1.8	9.0	32.7	1.7	8.7	31.0
Standard deviation	0.1	0.4	2.2	0.1	0.4	1.0
Coefficient of variation（%）	3.3	4.8	6.8	5.8	4.3	3.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human ICAM-1/CD54 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 83% to 120% with an overall mean recovery of 105%.

Sample Values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

55.22-176.34

100

103.75

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human ICAM-1/CD54 in this assay. No medical histories were available for the donors.

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