Human TNF R1/TNFRSF1A ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.01 ng/ml (50 μl)；0.13 ng/ml (10 μl)
Standard Curve Range: 0.07~50 ng/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0128

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Size:

96T

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Product Details

Background

Tumor necrosis factors (TNFs) are pleiotropic cytokines that are considered primary modifiers of the inflammatory and immune reactions of animals produced in response to injury or infection. Two forms of TNF, designated TNF-alpha (or cachectin) and TNF-beta (or lymphotoxin), have been described that share 30% sequence similarity and compete for binding to the same receptors. TNFs play a necessary and beneficial role as mediators of host resistance to infections and tumor formation. However, over-production or inappropriate expression of these factors can lead to a variety of pathological conditions, including wasting, systemic toxicity, and septic shock.

Typical data

ng/ml	O.D.		Average	Corrected
0.00	0.0187	0.0199	0.0193
0.07	0.0329	0.0316	0.0323	0.0130
0.21	0.0593	0.0605	0.0599	0.0406
0.62	0.1474	0.1490	0.1482	0.1289
1.85	0.3976	0.4073	0.4025	0.3832
5.56	1.0320	1.0090	1.0205	1.0012
16.67	2.1240	2.1440	2.1340	2.1147
50.00	3.4340	3.3810	3.4075	3.3882

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（ng/ml）	1.1	5.9	17.9	1.1	5.4	16.8
Standard deviation	0.0	0.3	1.3	0.0	0.2	0.5
Coefficient of variation（%）	4.1	5.7	7.0	3.9	3.1	2.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human TNF RI/TNFRSF1A into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 96% to 128% with an overall mean recovery of 120%.

Sample Values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

2.4-5.14

100

3.87

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated forthe presence of human TNF RI/TNFRSF1A in this assay. No medical histories were available for the donors.

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