IDH1 R132H Rabbit Monoclonal Antibody(ARB971)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6763
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. The high frequency and distribution of the IDH1 R132H mutation among specific brain tumor entities allow the highly sensitive and specific discrimination of various tumors by immunohistochemistry, such as anaplastic astrocytoma from primary glioblastoma or diffuse astrocytoma WHO grade II from pilocytic astrocytoma or ependymoma. Noteworthy is the discrimination of the infiltrating edge of tumors with IDH1 mutation from reactive gliosis. This antibody is highly useful for tumor classification and in detecting single infiltrating tumor cells. The routine practical approach for diagnosing astrocytomas and oligodendrogliomas begins with performing IHC for IDH1 R132H and ATRX expression.
Application
|
Application |
Dilution Ratio |
|
IHC |
1:100 - 1:200 |
Overview
|
Predicted Molecular Wt |
47kDa |
|
Species Cross-reactivity |
Human |
|
Applications |
IHC-P |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
Q75874 |
|
Subcellular location |
Cytoplasm |
|
Recommended method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Data
Immunohistochemical staining of human glioma tissue with IDH1-R132H mutation using IDH1 R132H Rabbit Monoclonal Antibody(ARB971)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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