IDH1 Rabbit Monoclonal Antibody(ARA946)
Key features and details
- Reactivity:Human
- Application:IHC
- Host:Rabbit
- Clonality:Monoclonal
- Target:IDH1
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Brand:
CAT.NO. : ARA6787
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Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Isocitrate dehydrogenase 1 (NADP+), soluble is an enzyme that in humans is encoded by the IDH1 gene on chromosome 2. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which uses NAD+ as the electron acceptor and the other NADP+. Five isocitrate dehydrogenases have been reported: three NAD+-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP+-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP+-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2,4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
1:200-1:3000 |
Overview
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Antibody Type |
Recombinant Rabbit Monoclonal Antibody |
|
Immunogen |
Synthetic peptide within human IDH1 aa 51 - 100 / 414 |
|
Species Reactivity |
Human, Mouse, Rat |
|
Validated Applications |
WB, IHC-P, IP |
|
Molecular Weight |
Predicted band size: 47 kDa |
|
Positive Control |
HeLa cell lysate, Raji cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue |
|
Conjugation |
unconjugated |
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Form |
Liquid |
|
Concentration |
1μg/ul |
|
Storage Buffer |
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified |
Data
Immunohistochemical staining of human prostate cancer tissue using IDH1 Rabbit Monoclonal Antibody(ARA946)
Immunohistochemical staining of human kidney tissue using IDH1 Rabbit Monoclonal Antibody(ARA946)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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