Isotype Controls

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Isotype Controls

An isotype control antibody, a primary antibody used as a negative control, helps determine the non - specific background staining level in an experiment.

Product Category

Antibodies
- Primary antibodies
- Secondary antibodies
- Antibody Pairs and Panels
- Isotype Controls
- Antigen & Cell Lysate
- Reagents
Kits
- ELISA Kit
- arigoQIK ELISA
- Multiplex ELISA Kits
- Assay Kit
Recombinant Proteins
Application
- WB
- ELISA
- IHC
- IP/ChIP
- ICC/IF
- FACS
- IHC-P
- IHC-Fr
- IHC-FoFr
- IHC-R
- IHC-Wmt
- IP
- ChIP
- ChIP-seq
- R-IP A
- ICC
- sELISA
- I-ELISA
- Agg
- Binding
- BL
- CCIe
- Cell-Act
- CLIA
- Confocal
- Conj
- Control
- CyTOF®-candidate
- Depletion
- Dot
- EIA
- EM
- EMSA
- ELISPOT
- FLISA
- FRET
- Functional Assay
- FuncSt
- GSA
- IA
- ID
- IE
- lfa
- Inhib
- ISH
- MeDIP
- Neut
- Puri
- RIA
- SDS-PAGE
Research Areas
- 基础科学研究
- 动物科学
- 生物制药
- 临床与诊断
Brand
- AREX
- arigo
- RevMAb
Host
- Rabbit
- Mouse
- Chicken
- Goat
- Human
- Donkey
- Guinea pig
- Hamster
- Llama
- Rhesus Monkey
- Syrian Hamster
Reactivity
- Human
- Ms
- Rat
- Cow
- Goat
- Mk
- Pig
- Rabbit
- Sheep
- AGMK
- Amph
- Arabi
- Bacteria
- Bb
- Bird
- Bov
- Camel
- Carp
- Cat
- Ce
- Cfsh
- Chem
- Chimp
- Chk
- Ctl
- Cucumber
- Cydippids
- C. albicans
- C. reinhardtii
- Deer
- Dlph
- Dm
- DEN
- Dog
- Duck
- D. discoideum
- EBV
- Elp
- Eisenia
- E. coli
- Fsh
- Fungi
- Firef
- Frt
- Glla
- Gpig
- Grb
- HBV
- HCV
- HIV
- Hm
- HPV
- Hrs
- H. pylori
- Influenza A
- Influenza B virus
- Insect
- Invt
- Macaq
- Mamm
- Marmoset
- M. tuberculosis
- NHuPrm
- Nicotiana
- Opossum
- Owl
- Paramecium
- Pgn
- Plnt
- Prt
- P. pastoris
- Rb
- Rice
- R. Mk
- Snk
- Shark
- S. aureus
- S. cerevisiae
- S. pombe
- Tetrahymena
- Turkey
- Virus
- Xenopus
- Xenopus laevis
- Yeast
- Zfsh
- Other
- All
- Animals

Function:

It differentiates between non - specific background and specific antibody signals. It indicates whether a sample has proper blocking and washing or needs further optimization.

Characteristics:

It has the same immunoglobulin isotype and subclass as the primary antibody but lacks target specificity. It's specific to a known irrelevant target like keyhole limpet hemocyanin (KLH).

Applications:

Mainly used in flow cytometry, it can also be a blocking or protein - coating agent in immunofluorescence, immunocytochemistry, Western blotting, and ELISA.

Selection:

When selecting an isotype control, it should match the primary antibody's host species and Ig class.

Popular Products

Related Application

ELISA

IHC

IP/ChIP

ICC/IF

FACS

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Room 315, West Zone, Building A, No. 525 Xixi Road, Xihu District, Hangzhou, China 310013

info@arexbio.com

400-886-0853

0571-8708-1890

0571-8708-2185

Products Antibodies - Primary antibodies - Secondary antibodies - Antibody Pairs and Panels - Isotype Controls - Antigen & Cell Lysate - Reagents Kits - ELISA Kit - arigoQIK ELISA - Multiplex ELISA Kits - Assay Kit Recombinant Proteins

Research Areas 基础科学研究动物科学生物制药临床与诊断

Application WB ELISA IHC IP/ChIP ICC/IF FACS

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