MSH2 Mouse Monoclonal Antibody(ARB849)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6639
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Product Details
Background
MSH2 is a 105 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 antibody gene is one of only 4 known to encode proteins involved in the repair of mismatch nucleotides following DNA replication or repair. . Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of hereditary nonpolyposis colorectal cancer (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
Overview
| Target | MSH2 |
| Host Species | mouse monoclonal antibody |
| Molecular Weight | 105 kDa |
| Purity | ProG affinity purified IgG |
| Species Cross-reactivity | Human |
| Form | Liquid |
| Applications | IHC-P |
| Swissprot ID | P43246 |
| Immunogen | Recombinant fragment of human MSH2 protein |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
| Storage Conditions | -25°C to -18°C |
| Dilutions | IHC-P: 1:100-1:200 |
| Subcellular Location | Nucleus |
| Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), RT for 30 minutes |
Data
Immunohistochemical staining of human tonsillar tissue sections using MSH2 Mouse Monoclonal Antibody (ARB849).
Immunohistochemical staining of human colorectal carcinoma tissue sections using MSH2 Mouse Monoclonal Antibody (ARB849).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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