Phospho-PI3K p85/p55 (Tyr467, Tyr199) Rabbit Polyclonal Antibody
Datasheet
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, ICC/IF, IHC(P)
- Host:Rabbit
- Clonality: Ponoclonal
- lsotype:IgG
- Target:Phospho-PI3K p85/p55
-
Brand:
CAT.NO. : ARA6611
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Product Details
Background
Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.
Overview
|
Species Reactivity |
Human, Mouse, Rat |
|
Host/Isotype |
Rabbit / IgG |
|
Class |
Polyclonal |
|
Type |
Antibody |
|
Conjugate |
Unconjugated |
|
Immunogen |
A synthesized peptide derived from human PIK3R1(Accession P27986), corresponding to amino acid residues around phosphorylated Tyr199. |
|
Form |
Liquid |
|
Concentration |
1 mg/mL |
|
Purification |
sequential chromatography |
|
Storage buffer |
PBS, pH 7.4, with 50% glycerol |
|
Contains |
0.02% sodium azide |
Application
|
Applications |
Tested Dilution |
|
Western Blot (WB) |
1:500 - 1:2,000 |
|
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50 - 1:200 |
|
Immunocytochemistry (ICC/IF) |
1:100 - 1:500 |
Data
Western blot: Extracts from 293 cells untreated or treated with sorbitol stained with anti - PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody.
Immunocytochemical analysis of Phospho - PI3K p85/p55 (Tyr458)/p55 (Tyr199) in NIH/3T3 cells using a Phospho - PI3K p85/p55 (Tyr458)/p55 (Tyr199) polyclonal antibody. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1:400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1:600 was used as secondary antibody.
Immunohistochemical analysis of Phospho - PI3K p85/p55 (Tyr458)/p55 (Tyr199) in Mouse stomach tissue using a Phospho - PI3K p85/p55 (Tyr458)/p55 (Tyr199) polyclonal antibody . The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti - Rabbit antibody was used as the secondary antibody.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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