PI3K p85 alpha Mouse Monoclonal Antibody（ARA820）
Datasheet
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IP, ICC/IF, IHC(P)
- Host:Mouse
- Clonality: Monoclonal
- lsotype:IgG1
- Target:PI3K p85 alpha
-
Brand:
CAT.NO. : ARA6610
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Product Details
Background
Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.
Overview
|
Species Reactivity |
Human, Mouse, Rat |
|
Host/Isotype |
Mouse / IgG1 |
|
Class |
Monoclonal |
|
Conjugate |
Unconjugated |
|
Immunogen |
Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724. |
|
Form |
Liquid |
|
Concentration |
1 mg/mL |
|
Purification |
Protein A |
|
Storage buffer |
PBS |
|
Contains |
0.09% sodium azide |
Application
|
Applications |
Tested Dilution |
|
Western Blot (WB) |
1:100 - 1:2000 |
|
Immunohistochemistry (Frozen) (IHC (F)) |
1:50 - 1:500 |
|
Immunocytochemistry (ICC/IF) |
1:50 - 1:200 |
|
Immunoprecipitation (IP) |
- |
Data
Immunohistochemistry analysis of PI3-kinase p85 subunit alpha in paraffin-embedded rat brain tissue. Samples were incubated with PI3-kinase p85 subunit alpha monoclonal antibody (ARA820), and followed by hematoxylin.
Immunohistochemical analysis of paraffin - embedded human lung tissue with Mouse anti - PI 3 Kinase p85 alpha antibody at 1/1,000 dilution.
Immunofluorescent analysis of PI3-kinase p85 subunit alpha in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PI3-kinase p85 subunit alpha monoclonal antibody (ARA820), followed by DAPI (blue).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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