PMS2 Rabbit Monoclonal Antibody(ARB844)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6634
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Product Details
Background
Mismatch repair (MMR) proteins is a group of nuclear enzymes, which in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MMR proteins leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI).
The PMS2 protein forms a heterodimer with the MLH1 protein which is then activated in the presence of ATP; this complex coordinates the binding of other proteins that repair DNA errors arising during cell preparation for cell division. The loss of PMS2 expression in tumors can be helpful in identifying hMLH1 mutation carriers and identifies their suitability for mutation analysis. PMS2 gene defects account for a small but significant proportion of colorectal cancers and for a substantial proportion of tumors with microsatellite instability.
Compared to molecular biological techniques, immunohistochemical analysis of MMR protein expression is much simpler and cheaper. Immunohistochemical analysis helps to pinpoint the affected gene and should be readily accessible in a pathology laboratory.
The PMS2 protein forms a heterodimer with the MLH1 protein which is then activated in the presence of ATP; this complex coordinates the binding of other proteins that repair DNA errors arising during cell preparation for cell division. The loss of PMS2 expression in tumors can be helpful in identifying hMLH1 mutation carriers and identifies their suitability for mutation analysis. PMS2 gene defects account for a small but significant proportion of colorectal cancers and for a substantial proportion of tumors with microsatellite instability.
Compared to molecular biological techniques, immunohistochemical analysis of MMR protein expression is much simpler and cheaper. Immunohistochemical analysis helps to pinpoint the affected gene and should be readily accessible in a pathology laboratory.
Overview
| Target | PMS2 |
| Host Species | rabbit monoclonal antibody |
| Molecular Weight | 96 kDa |
| Purity | ProA affinity purified IgG |
| Species Cross-reactivity | Human |
| Form | Liquid |
| Applications | IHC-P |
| Swissprot ID | P54278 |
| Immunogen | Synthetic peptide corresponding to residues within aa1-100 of PMS2 |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
| Storage Conditions | -25°C to -18°C |
| Dilutions | IHC-P: 1:100-1:200 |
| Subcellular Location | Nucleus |
| Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes |
Data
Immunohistochemical staining of human esophageal tissue sections for PMS2 using PMS2 Rabbit Monoclonal Antibody (ARB844).
Immunohistochemical staining of human renal tissue sections for PMS2 using PMS2 Rabbit Monoclonal Antibody (ARB844).
Immunohistochemical staining of human colonic tissue sections for PMS2 using PMS2 Rabbit Monoclonal Antibody (ARB844).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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