Prohibitin Rabbit Monoclonal Antibody(ARA729)

Key features and details

  • Reactivity:Human, Mouse, Rat
  • Application:WB, IF/ICC, IHC, IP, FC
  • Host:Rabbit
  • Clonality:Monoclonal
  • Target:Prohibitin
  • Brand:
CAT.NO. : ARA6516
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Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Application

Application

Dilution Ratio

WB

1:500 - 1:1000

IF/ICC

1:100 - 1:1000

IHC

1:200 - 1:1000

IP

1-2μg/sample

FC

1:800 - 1:1000

Overview

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Prohibitin aa 147-196 / 272.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF/ICC, IHC, IP, FC

Molecular Weight

Predicted band size: 30 kDa

Positive Control

HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, hybrid fish (crucian-carp) liver tissue lysates, HepG2, PC-12, NIH/3T3, human liver carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, mouse stomach tissue, mouse heart tissue, human liver tissue.

Conjugation

unconjugated

RRID

AB_3069644

Form

Liquid

Storage Buffer

1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Data

Western blot analysis of Prohibitin on different lysates with Prohibitin Rabbit Monoclonal Antibody(ARA729).
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Prohibitin Rabbit Monoclonal Antibody(ARA729).
Prohibitin was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Prohibitin Rabbit Monoclonal Antibody(ARA729).
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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