Recombinant Apis Mellifera Phospholipase A2/Api m1

Key features and details

  • Species:
  • Abbreviation: rApi m1
  • Source: Expressed in E. coli.
  • Molecular Weight: Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
  • Purity: >97% by SDS-PAGE or HPLC.
  • Endotoxin: < 0.01 EU/μg of rApi m1 protein as determined by LAL method.
  • Accession #: P00630 Ile34-Tyr167
  • Storage:
  • Brand:
CAT.NO. : ARP6804
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Product Details
Background
Api m1, a glycoprotein of the phospholipase 2 (PLA2) family, is a major allergen of Apis mellifera  (honeybee) venom (HBV) and a marker allergen for genuine sensitization to this venom. Api m 1 contributes 12-16% of venom dry weight. Api m 1 is consistently found as a major allergen in HBV-allergic patients. Of the 12 known allergens within HBV, Api m 1 is considered the most prominent allergen, in terms of prevalence of sensitization, levels of specific IgE to Api m 1, and quantitative correlation between Api m 1 and HBV specific IgE. Api m 1 does not cross-react with phospholipases of the PLA1 family found in Vespid venoms, therefore, Api m 1 is a marker allergen allowing to discriminate between HBV and Vespid venom sensitization. Recombinant Api m1 is a 15.2kDa globular protein containing 134 amino acid residues.
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