Succinyllysine Rabbit Polyclonal Antibody
Key features and details
- Reactivity:All
- Application:WB
- Host:Rabbit
- Clonality:polyclonal
- Target:Succinyllysine
-
Brand:
CAT.NO. : ARA6483
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Size:
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Product Details
Background
Succinylation is a post-translational modification that involves the addition of a succinyl group (-CO-CH2-CH2-CO2 h) is added to a lysine residue of a protein molecule. This modification is found in many proteins, including histones. Although the precise implications of succinylation are under investigation, due to its impact on lysine's charge, transitioning it from +1 to -1 (under physiological pH conditions), and because succinylation introduces a relatively substantial structural moiety with a mass of 100 Da, which is notably larger than the moieties introduced by acetylation (42 Da) or methylation (14 Da), succinylation is anticipated to induce more substantial alterations in both protein structure and function. Key players in the succinylation process comprise 'writers' such as carnitine palmitoyltransferase 1A (CPT1A) and lysine acetyltransferase 2A (KAT2A/GCN5), 'erasers' like sirtuin 5 (SIRT5) and sirtuin 7 (SIRT7), and the pH-dependent reader, the YEATS domain of glioma-amplified sequence 41 (GAS41).
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
Overview
|
Isotype |
IgG |
|
Conjugate |
Unconjugated |
|
Synonyms |
Ksu |
|
Immunogen |
Succinylated lysine peptides |
|
Molecular Weight (MW) |
Multiple |
|
Specificity |
Anti-Succinyllysine Rabbit pAb detects proteins post-translationally modified by succinylation on lysine residues. This pan antibody recognizes succinylated lysine independent of its surrounding sequences. |
|
Host |
Rabbit |
|
Clonality |
Polyclonal |
|
Reactivity |
All |
|
Purity |
Protein A and immunogen affinity purified |
|
Constituents |
PBS, Glycerol, BSA |
|
Stability |
Stable for 12 months from date of receipt/reconstitution. |
Data
Lysates: E. coli, S. cerevisiae, D. melanogaster, mouse, and human cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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