anti-ARF1 antibody [1B3]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [1B3] recognizes ARF1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1B3 |
| 同位型 | IgG1, kappa |
| 靶点名称 | ARF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-181 of Human ARF1 |
| 偶联标记 | Un-conjugated |
| 別名 | ADP-ribosylation factor 1 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | ARF1 |
| 全名 | ADP-ribosylation factor 1 |
| 背景介绍 | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3. |
ARG57110 anti-ARF1 antibody [1B3] ICC/IF image
Immunofluorescence: MCF7 cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57110 anti-ARF1 antibody [1B3] FACS image
Flow Cytometry: MCF7 cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57110 anti-ARF1 antibody [1B3] WB image
Western blot: 40 µg of 1) HeLa, 2) HepG2, 3) Brain tissue lysate, and 4) MCF cell lysates stained with ARG57110 anti-ARF1 antibody [1B3] at 1:1000.
ARG57110 anti-ARF1 antibody [1B3] ICC/IF image
Immunofluorescence: U87MG cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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