SOX9 Rabbit Monoclonal Antibody(ARA829)

Key features and details

Reactivity:Human, Mouse, Rat
Application:WB, IF-Cell, IHC-P, IP, FC, CUT&Tag-seq, mIHC
Host:Rabbit
Clonality:Monoclonal
Target:SOX9

Brand:

CAT.NO. : ARA6632

RMB Please choose

Size:

1mL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

Application

Application	Dilution Ratio
IHC-P	1:200-1:1000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within human SOX9 aa 140-340
Species Reactivity	Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Validated Applications	WB, IF-Cell, IHC-P, IP, FC, CUT&Tag-seq, mIHC
Molecular Weight	Predicted band size: 56 kDa
Positive Control	SW480 cell lysate, MDA-MB-231 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, MDA-MB-231, human tonsil tissue, human breast carcinoma tissue, mouse liver tissue, human colon tissue, human thyroid tissue, human breast tissue, human small intestine tissue, human prostate carcinoma tissue, human stomach carcinoma tissue, human lung carcinoma tissue, rat colon tissue
Conjugation	unconjugated
Form	Liquid
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified

Data

Immunohistochemical staining of human tonsil tissue sections using SOX9 Rabbit Monoclonal Antibody (ARA829).

Immunohistochemical staining of human breast carcinoma tissue sections using SOX9 Rabbit Monoclonal Antibody (ARA829).

Immunohistochemical staining of human colon tissue sections using SOX9 Rabbit Monoclonal Antibody (ARA829).

Immunohistochemical staining of human prostate carcinoma tissue sections using SOX9 Rabbit Monoclonal Antibody (ARA829).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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