anti-ATG12 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ATG12
  • 反应物种: Hu
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATG12
  • 抗原物种: Human
  • 抗原: KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human ATG12.
  • Brand:
CAT.NO. : ARG56217
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ATG12
反应物种Hu
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ATG12
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human ATG12.
偶联标记Un-conjugated
別名Ubiquitin-like protein ATG12; FBR93; HAPG12; APG12-like; Autophagy-related protein 12; APG12L; APG12
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200
IHC-P1:50 - 1:100
WB1:1000 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照A549
属性
形式Liquid
纯化This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液PBS and 0.09% (W/V) Sodium azide.
抗菌剂0.09% (W/V) Sodium azide.
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 9140 Human ATG12

Swiss-port # O94817 Human Ubiquitin-like protein ATG12

基因名称ATG12
全名autophagy related 12
背景介绍Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
生物功能Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. [UniProt]
细胞定位Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane
预测分子量15 kDa
翻译后修饰Acetylated by EP300.
检测图片 (3)
  • ARG56217 anti-ATG12 antibody ICC/IF image

    Immunofluorescence: U251 cells were treated with Chloroquine (50 µM, 16 hours), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then stained with ARG56217 anti-ATG12 antibody (green) at 1:200 dilution, 2 hours at room temperature. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).

  • ARG56217 anti-ATG12 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG56217 anti-ATG12 antibody.

  • ARG56217 anti-ATG12 antibody WB image

    Western blot: 20 µg of A549 cell lysate stained with ARG56217 anti-ATG12 antibody at 1:2000 dilution.

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