anti-ATP6V1G3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP6V1G3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP6V1G3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 15-49 of Human ATP6V1G3. |
| 偶联标记 | Un-conjugated |
| 別名 | ATP6G3; Vacuolar proton pump subunit G 3; Vma10; V-ATPase subunit G 3; V-type proton ATPase subunit G 3; V-ATPase 13 kDa subunit 3 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human kidney |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | ATP6V1G3 |
| 全名 | ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3 |
| 背景介绍 | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProt] |
| 预测分子量 | 14 kDa |
ARG40147 anti-ATP6V1G3 antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells stained with ARG40147 anti-ATP6V1G3 antibody (green) at 1:25 dilution. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). DAPI (blue) for nuclear staining.
ARG40147 anti-ATP6V1G3 antibody WB image
Western blot: 20 µg of Human kidney lysate stained with ARG40147 anti-ATP6V1G3 antibody at 1:2000 dilution.
ARG40147 anti-ATP6V1G3 antibody FACS image
Flow Cytometry: U-2 OS cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG40147 anti-ATP6V1G3 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
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