anti-CD63 antibody [MX-49.129.5]

Key features and details

产品描述: Mouse Monoclonal antibody [MX-49.129.5] recognizes CD63
反应物种: Hu, Ms
应用: FACS, ICC/IF, IHC-P, WB
宿主: Mouse
克隆: Monoclonal
克隆号: MX-49.129.5
同位型: IgG1, kappa
靶点名称: CD63
抗原物种: Human

Brand:

CAT.NO. : ARG41312

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Size:

50 μg

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [MX-49.129.5] recognizes CD63
反应物种	Hu, Ms
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	MX-49.129.5
同位型	IgG1, kappa
靶点名称	CD63
抗原物种	Human
抗原	Full length Human CD63.
偶联标记	Un-conjugated
別名	Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 2 µg/10^6 cells
ICC/IF	1 - 5 µg/ml
IHC-P	1 - 5 µg/ml
WB	1 - 2 µg/ml

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂	0.05% Sodium azide
稳定剂	0.1 mg/ml BSA
浓度	0.2 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 12512 Mouse CD63 GeneID: 967 Human CD63 Swiss-port # P08962 Human CD63 antigen Swiss-port # P41731 Mouse CD63 antigen
基因名称	CD63
全名	CD63 molecule
背景介绍	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
生物功能	Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
细胞定位	Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, exosome. Cell surface. Note=Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903). [UniProt]
产品亮点	Related products: CD63 antibodies; Anti-Mouse IgG secondary antibodies; Related news: New antibodies for exosome isolation
预测分子量	26 kDa
翻译后修饰	Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). [UniProt]

检测图片 (7)

ARG41312 anti-CD63 antibody [MX-49.129.5] ICC/IF image
Immunofluorescence: HeLa cells stained with Alexa Fluor 488-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (green). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI (blue) for nuclear staining.
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image
Immunohistochemistry: Paraffin-embedded Human melanoma tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] WB image
Western blot: Human spleen lysate stained with ARG41312 anti-CD63 antibody [MX-49.129.5] at 2 µg/ml dilution.
ARG41312 anti-CD63 antibody [MX-49.129.5] FACS image
Flow Cytometry: Human PBMC cells stained with PE-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (red). Cells alone (black). Isotype control (green).
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate carcinoma tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] FACS image
Flow Cytometry: NIH/3T3 cells stained with PE-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (red). Cells alone (black). Isotype control (green).