anti-DDX58 / RIGI antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG54925
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DDX58 / RIGI |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DDX58 / RIGI |
| 抗原物种 | Human |
| 抗原 | GST-tagged Human RIG-1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | RIGI; RIG-I-like receptor 1; RIG-I; SGMRT2; Probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; DEAD box protein 58; EC 3.6.4.13; Retinoic acid-inducible gene I protein; RIG-1; RLR-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | C2C12 Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O95786 Human Probable ATP-dependent RNA helicase DDX58 Swiss-port # Q6Q899 Mouse Probable ATP-dependent RNA helicase DDX58 |
|---|---|
| 基因名称 | DDX58 |
| 全名 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| 背景介绍 | RIG-1 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus. |
| 生物功能 | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA ( |
| 产品亮点 | Related products: DDX58 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 107 kDa |
| 翻译后修饰 | Phosphorylated in resting cells and dephosphorylated in RNA virus-infected cells. Phosphorylation at Thr-770, Ser-854 and Ser-855 results in inhibition of its activity while dephosphorylation at these sites results in its activation. ISGylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation. ISGylation negatively regulates its function in antiviral signaling response. Sumoylated, probably by MUL1; inhibiting its polyubiquitination. Ubiquitinated. Undergoes 'Lys-48'- and 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS/IPS1 binding and to induce anti-viral signal transduction (PubMed:17392790). Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated and TRIM4-mediated ubiquitination (PubMed:19017631, PubMed:19484123, PubMed:24755855). Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains (PubMed:18636086). Also probably deubiquitinated by USP17L2/USP17 that cleaves 'Lys-48'-and 'Lys-63'-linked ubiquitin chains and positively regulates the receptor (PubMed:20368735). Ubiquitinated at Lys-181 by RNF125, leading to its degradation: ubiquitination takes place upon viral infection and is enhanced 'Lys-63'-linked ubiquitination of the CARD domains, which promote interaction with VCP/p97 and subsequent recruitment of RNF125 (PubMed:17460044, PubMed:26471729). |
检测图片 (2)
ARG54925 anti-DDX58 / RIGI antibody WB image
Western blot: C2C12 cell lysate stained with ARG54925 anti-DDX58 / RIGI antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml dilution.
ARG54925 anti-DDX58 / RIGI antibody IHC image
Immunohistochemistry: RIG-1 in Human heart tissue stained with ARG54925 anti-DDX58 / RIGI antibody at 5 ug/ml dilution.
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