Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-00
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CAT.NO. : 31-1048-00
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Product Details
Product Details
| Product Name | Anti-His-Tag (6x His-Tag or 10 xHis-Tag) Rabbit Monoclonal Antibody, Clone RM146 |
| Product Description | Rabbit monoclonal to His-Tag. (6x His-Tag or 10x His-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy) |
| Catalog No. | 31-1048-00 |
| Clone Name | RM146 |
| Product Data Sheet | Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146 |
| Specificity | RM146 reacts to recombinant proteins containing the 6x His-Tag or 10x His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. |
| Immunogen | Mixture of a peptide with 6x His-Tag at the N-terminus and another peptide with 6x His-Tag at the C-terminus. |
| Application | Western Blot Immunoprecipitation Immunocytochemistry Flow Cytometry Immunohistochemistry |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| Related Products | For N-terminal His-tagged protein detection, RM146X is recommended. Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.1 µg/mL – 1 µg/mL; Immunoprecipitation: 0.5 µg/mL – 2 µg/mL; Immunocytochemistry 0.5 µg/mL – 2 µg/mL; Flow Cytometry: 0.5 µg/mL – 2 µg/mL; Immunohistochemistry; 0.1 µg/mL-1 µg/mL. |
| References for 6x His-Tag or 10x His-Tag antibody (RM146) | Goto S et al Enhanced anti-tumor efficacy of IL-7/CCL19-producing human CAR-T cells in orthotopic and patient-derived xenograft tumor models. Cancer Immunol Immunother. (2021), 10.1007/s00262-021-02853-3. FC; Human. Read more (PubMed: 33559069) |
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Product Images
 | Western blot of 293T cells: (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y Using RevMAb Clone RM146 at 0.2 µg/mL. |
 | Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by RevMAb Clone RM146, was blotted with RM146. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM146. |
 | Immunohistochemistry staining of naïve HepG2 cells (Negative control) using anti-His-Tag antibody, Clone RM146. |
 | Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, Clone RM146. |
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