anti-IL2 antibody [SQab18130]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [SQab18130] recognizes IL2
  • 反应物种: Hu
  • 应用: ELISA, FACS, ICC/IF, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: SQab18130
  • 同位型: IgG1, kappa
  • 靶点名称: IL2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66428
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Product Details
概述
产品描述Mouse Monoclonal antibody [SQab18130] recognizes IL2
反应物种Hu
应用ELISA, FACS, ICC/IF, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号SQab18130
同位型IgG1, kappa
靶点名称IL2
抗原物种Human
抗原Recombinant Human IL2 protein.
偶联标记Un-conjugated
別名TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor
应用说明
应用建议
应用推荐稀释比
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:400 - 1:1000
IHC-PAssay-dependent
WB1:2500 - 1:5000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂0.01% Thimerosal
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3558 Human IL2

Swiss-port # P60568 Human Interleukin-2

基因名称IL2
全名interleukin 2
背景介绍The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
生物功能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
细胞定位Secreted. [UniProt]
预测分子量18 kDa
检测图片 (4)
  • ARG66428 anti-IL2 antibody [SQab18130] ICC/IF image

    Immunofluorescence: MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66428 anti-IL2 antibody [SQab18130] (green) at 1:400 and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66428 anti-IL2 antibody [SQab18130] FACS image

    Flow Cytometry: MCF-7 cells were stained with ARG66428 anti-IL2 antibody [SQab18130] at 1 µg/ml (red) and without antibody control (black).

  • ARG66428 anti-IL2 antibody [SQab18130] WB image

    Western blot: 100 ng of Recombinant IL2 protein and 60 µg of MCF-7 cell lysate stained with ARG66428 anti-IL2 antibody [SQab18130] at 1:2000 dilution.

  • ARG66428 anti-IL2 antibody [SQab18130] ELISA image

    ELISA: Titration curve of ARG66428 anti-IL2 antibody [SQab18130]. Red: IL2; Black: BSA (negative control).

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