anti-Ku 70 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG57851
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Ku 70 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Ku 70 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human Ku70. |
| 偶联标记 | Un-conjugated |
| 別名 | DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70 |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | SW480 | ||||||||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P12956 Human X-ray repair cross-complementing protein 6 Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6 |
|---|---|
| 基因名称 | XRCC6 |
| 全名 | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| 背景介绍 | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] |
| 生物功能 | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt] |
| 细胞定位 | Chromosome, Nucleus. [UniProt] |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. [UniProt] |
检测图片 (3)
ARG57851 anti-Ku 70 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG57851 anti-Ku 70 antibody.
ARG57851 anti-Ku 70 antibody WB image
Western blot: 25 µg of SW480 cell lysate stained with ARG57851 anti-Ku 70 antibody at 1:1000 dilution.
ARG57851 anti-Ku 70 antibody IP image
Immunoprecipitation: 100 µg extracts of SW480 cells were immunoprecipitated and stained with ARG57851 anti-Ku 70 antibody at 1:1000 dilition.
参考文献
miR-3059-3p Regulates Glioblastoma Multiforme Radiosensitivity Enhancement through the Homologous Recombination Pathway of DNA Repair
WB / Human
Phospho-Ku70 induced by DNA damage interacts with RNA Pol II and promotes the formation of phospho-53BP1 foci to ensure optimal cNHEJ
WB / Human
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