anti-Ly6C antibody [ER-MP20]

Key features and details

  • 产品描述: Rat Monoclonal antibody [ER-MP20] recognizes Ly6CRat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
  • 反应物种: Ms
  • 应用: FACS, IHC-Fr, IP
  • 宿主: Rat
  • 克隆: Monoclonal
  • 克隆号: ER-MP20
  • 同位型: IgG2a
  • 靶点名称: Ly6C
  • 抗原物种: Mouse
  • Brand:
CAT.NO. : ARG22862
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Product Details
概述
产品描述Rat Monoclonal antibody [ER-MP20] recognizes Ly6C
Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
反应物种Ms
应用FACS, IHC-Fr, IP
宿主Rat
克隆Monoclonal
克隆号ER-MP20
同位型IgG2a
靶点名称Ly6C
抗原物种Mouse
抗原Balb/c macrophage precursor cell hybrids.
偶联标记Un-conjugated
別名AA959465; Ly-6C1; AA682074; Ly-6C; Lymphocyte antigen 6C1; Ly6c
应用说明
应用建议
应用推荐稀释比
FACSNeat
IHC-FrAssay-dependent
IPAssay-dependent
应用说明FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS and 0.09% Sodium azide
抗菌剂0.09% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 17067 Mouse Ly6c1

Swiss-port # P0CW02 Mouse Lymphocyte antigen 6C1

基因名称Ly6c1
全名lymphocyte antigen 6 complex, locus C1
产品亮点Related products:
Ly6 antibodies; Ly6 ELISA Kits; Ly6 Duos / Panels; Anti-Rat IgG secondary antibodies;
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研究领域Mouse Inflammatory Cell Marker antibody; Neurophil Marker antibody; Mouse MDSC Marker antibody; Myeloid-derived suppressor cell antibody
预测分子量14 kDa
检测图片 (3)
  • ARG22862 anti-Ly6C antibody [ER-MP20] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22862 anti-Ly6C antibody [ER-MP20] followed by HRP-conjugated Goat anti Rat IgG antibody. (Low power).

  • ARG22862 anti-Ly6C antibody [ER-MP20] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22862 anti-Ly6C antibody [ER-MP20] followed by HRP-conjugated Goat anti Rat IgG antibody. (Medium power).

  • ARG22862 anti-Ly6C antibody [ER-MP20] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22862 anti-Ly6C antibody [ER-MP20] followed by HRP-conjugated Goat anti Rat IgG antibody. (High power).

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