Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [Clone RM195]; H4R3me1; Histone H4 (monomethyl Arg3); 31-1080-00; 31-1080-00-S
![Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [Clone RM195]; H4R3me1; Histone H4 (monomethyl Arg3); 31-1080-00; 31-1080-00-S](/upload/image/products/Fig-3_ICC_H4R3m1.png)
![Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [Clone RM195]; H4R3me1; Histone H4 (monomethyl Arg3); 31-1080-00; 31-1080-00-S](/upload/image/products/Fig-1_RM195_e.png)
![Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [Clone RM195]; H4R3me1; Histone H4 (monomethyl Arg3); 31-1080-00; 31-1080-00-S](/upload/image/products/Fig-2_RM195_e.png)
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CAT.NO. : 31-1080-00
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Product Details
Product Details
| Product Name | Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195 |
| Product Description | Rabbit monoclonal to Monomethylated Histone H4 Arginine 3, H4R3me1, Histone H4 (monomethyl R3) |
| Catalog No. | 31-1080-00 |
| Clone Name | RM195 |
| Product Data Sheet | Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195 |
| Specificity | RM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4. |
| Immunogen | A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL – 2 µg/mL; Immunocytochemistry: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.2 µg/mL – 1 µg/mL. |
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Product Images
 | RM195 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4. |
 | Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 µg/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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