anti-SQSTM1 / p62 antibody [3H11]

Key features and details

产品描述: Mouse Monoclonal antibody [3H11] recognizes SQSTM1 / p62
反应物种: Hu, Ms, Rat
应用: FACS, ICC/IF, IHC-P, WB
宿主: Mouse
克隆: Monoclonal
克隆号: 3H11
同位型: IgG2a
靶点名称: SQSTM1 / p62
抗原物种: Human

Brand:

CAT.NO. : ARG42026

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Size:

50 μg

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [3H11] recognizes SQSTM1 / p62
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	3H11
同位型	IgG2a
靶点名称	SQSTM1 / p62
抗原物种	Human
抗原	Synthetic peptide corresponding to aa. 69-96 of Human SQSTM1 / p62. (DEDGDLVAFSSDEELTMAMSYVKDDIFR)
偶联标记	Un-conjugated
別名	FTDALS3; p60; p62; EBI3-associated protein of 60 kDa; p62B; Sequestosome-1; Ubiquitin-binding protein p62; PDB3; OSIL; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBIAP; A170; ZIP3

应用说明

应用建议

应用	推荐稀释比
FACS	1:150 - 1:500
ICC/IF	1:200 - 1:1000
IHC-P	1:200 - 1:1000
WB	1:500 - 1:2000

应用说明

IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

~ 62 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂	0.05% Sodium azide
稳定剂	4% Trehalose
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 113894 Rat SQSTM1 GeneID: 18412 Mouse SQSTM1 GeneID: 8878 Human SQSTM1 Swiss-port # O08623 Rat Sequestosome-1 Swiss-port # Q13501 Human Sequestosome-1 Swiss-port # Q64337 Mouse Sequestosome-1 More
基因名称	SQSTM1
全名	sequestosome 1
背景介绍	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
生物功能	Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. [UniProt]
细胞定位	Cytoplasm, cytosol. Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus. Endoplasmic reticulum. Nucleus, PML body. Cytoplasm, myofibril, sarcomere. [UniProt]
产品亮点	Related products: SQSTM1 antibodies; SQSTM1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched Therapeutic strategies against PDAC
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
预测分子量	48 kDa
翻译后修饰	Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2 (PubMed:25040165). Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery (PubMed:27368102). [UniProt]

检测图片 (10)

ARG42026 anti-SQSTM1 / p62 antibody [3H11] ICC/IF image
Immunofluorescence: A431 cells stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] WB image
Western blot: 50 µg of samples under reducing conditions. CCRF-CEM, HepG2, HT1080 and SW620 whole cell lysates stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 0.5 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat liver, PC-12, RH35, Mouse brain, Mouse liver, NIH/3T3, Raw246.7 and Neuro-2a whole cell lysates stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 0.5 µg/ml dilution, overnight at 4°C.
ARG42026 anti-SQSTM1 / p62 antibody [3H11] FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.